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Acad. Sci. A. 79, 5956. Bech-Hansen, N. , and Cox, D. W. (1986). A m . J Hum. Genet. 38, 67. Becker, D. , Chien, Y. , Gascoigne, N. R. , Arai, K. , and Davis, M. M. (1985). Nature (London) 317, 430. Behlke, M. , Spinella, D. , Chou, H. S . , Hartl, D. , and b h , D. Y. (1985). Science 229, 566. Behlke, M. , Chou, H. S . , and Loh, D. Y. (1986). Proc. Natl. Acad. Sci. A. 83, 767. , and McDevitt, H. 0. (1972). Science 175, 273. Bentley, D. L. (1984). Nature (London) 307, 77. Bentley, D. , and Rabbitts, T.
And Alt, F. W. (1988). E M B O J. 7, 727. , and Krawinkel, U. (1987). Immunogenetics 26, 237. Blattner, F.
1987). , 1984). The class I1 regions are present on the centromeric side of the locus and are arranged in the order 5 ’-DPDQ-DR-3’ (Fig. 8a and b). , 1985) subregions have been isolated as genomic clones. However, efforts to link these subregions have been unsuccessful because of the large size of the locus and the frequency of repeat sequences, both of which make chromosomal “walking” difficult. The development of pulsed-field gel electrophoresis has permitted the rapid mapping and ordering of these subregions on the chromosome.